Human H (+)/Sialic Acid Cotransporter (AST) ELISA Kit

Cat.#:

JL18005

Regulatory Status:

RUO

Alternative:

AST;SLC17A5; SD; ISSD; NSD; ;SIASD; SLD; Solute Carrier Family 17 Member 5; Sialic Acid Storage Disease; Vesicular H(+)/Aspartate-glutamate cotransporter;Anion/Sugar Transporter ；H(+)/nitrate cotransporter and H(+)/sialic acid cotransporter

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| AST

Sialin, also known as H(+)/nitrate cotransporter and H(+)/sialic acid cotransporter, is a protein which is encoded by the SLC17A5 gene.A deficiency of this protein causes Salla disease and Infantile Sialic Acid Storage Disease (ISSD).The gene for HP59 contains, entirely within its coding region, the Sialin Gene SLC17A5. Member 5, also known as SLC17A5 or sialin is a lysosomal membrane sialic acid transport protein which in humans is encoded by the SLC17A5 gene on Chromosome 6.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.36	1.56	1.04	0.65	0.46	0.34	0.29	0.1
Corrected OD	2.26	1.46	0.94	0.55	0.36	0.24	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human AST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	90-105	102
Cell Lysates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human AST and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	86-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	109